期刊
FERTILITY AND STERILITY
卷 84, 期 6, 页码 1665-1673出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.fertnstert.2005.05.068
关键词
sperm maturity; chromosomal aneuploidies; ICSI sperm selection; genetic integrity; paternal contribution
资金
- NICHD NIH HHS [HD-19505] Funding Source: Medline
- NIOSH CDC HHS [OH-04061] Funding Source: Medline
Objective: To test a newly invented intracytoplasmic sperm injection (ICSI) sperm selection method based on sperm hyaluronic acid (HA) binding. Design: Comparison of chromosomal disomy and diploidy frequencies in sperm arising from semen and in HA-bound sperm. Setting: Academic andrology laboratory. Patient(s): Men presenting for semen analysis. Intervention(s): Washed sperm fractions of 32 semen samples were applied to Petri dishes or glass slides coated with immobilized HA. The unbound sperm were rinsed gently, and the HA-bound sperm were removed with an ICSI pipette. The control sperm population was the unselected sperm. Both HA-selected and unselected sperm were treated with fluorescence in situ hybridization with centromeric probes for the X, Y, and 17 chromosomes. Main Outcome Measure(s): Chromosomal disomy and diploidy frequencies. Result(s): In the HA-bound sperm (495-2,079 per man, 41,670 in all) compared with unselected sperm (4,770 per man, 162,210 in all), the chromosomal disomy frequencies were reduced to 0.16% from 0.52%, diploidy to 0.09% from 0.51%, and sex chromosome disomy to 0.05% from 0.27% (a 5.4-fold reduction vs. 4-fold respective increase in ICSI offspring). Conclusion(s): The HA sperm selection method for ICSI, which is based on a relationship between sperm receptors for zona pellucida and HA, will likely reduce the potential genetic complications and adverse public health effects of ICSI.
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