A method to size DNA fragments from 50 to 800 bp on a DNA analyser

We present a method to size DNA fragments from 50 to 800 bp on a DNA analyser using an alternative size standard (X-Rhodamine MapMarker 1000) run on a capillary electrophoresis system (Applied Biosystem's 3730 DNA Analyser). This method avoids several limitations imposed by manufacturer-developed size standards, instruments and software and offers a more robust approach to conducting DNA size fragment analyses.