期刊
WEED RESEARCH
卷 45, 期 6, 页码 413-423出版社
WILEY
DOI: 10.1111/j.1365-3180.2005.00473.x
关键词
genetic diversity; herbicide resistance; intersimple sequence repeat markers; long-primer random amplified polymorphic DNA; Avena fatua
Genetic diversity within and among 20 herbicide-resistant (HR) and 16 herbicide-susceptible (HS) Avena fatua multi-field populations was determined using 82 polymorphic loci resulting from two intersimple sequence repeat (ISSR) primers and one long-primer random amplified polymorphic DNA (LP-RAPD) primer. Collections from the Red River Valley of North Dakota and Minnesota, sampled in 1964 and 2000, represented A. fatua populations before and after intensive exposure to herbicides. A 1995 collection from south-west North Dakota represented A. fatua exposed to low herbicide selection. Despite differences in years of herbicide exposure among collections, both HR and HS populations from every collection maintained nearly similar levels of ISSR and RAPD diversity. Genetic differentiation among populations (G(ST)) varied from 11% to 13% among HR populations and from 9% to 16% among HS populations, indicating that 84-91% of total variation remained within HS or within HR populations. Minimal difference in gene diversity between HR and HS is consistent with multiple origins of resistance, where HR A. fatua most likely evolved from diverse founding individuals.
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