期刊
CANADIAN JOURNAL OF MICROBIOLOGY
卷 51, 期 12, 页码 1061-1069出版社
CANADIAN SCIENCE PUBLISHING
DOI: 10.1139/W05-094
关键词
plant growth-promoting bacteria; phytoremediation; copper; polycyclic aromatic hydrocarbons; Brassica napus; ethylene; alginate encapsulation
Growth of canola (Brassica napus) seeds treated with plant growth-promoting bacteria in copper-contaminated and polycyclic aromatic hydrocarbon (PAH)-contaminated soils was monitored. Pseudomonas asplenii AC, isolated from PAH-contaminated soil, was transformed to express a bacterial gene encoding 1-aminocyclopropane-1-carboxylate (ACC) deaminase, and both native and transformed bacteria were tested for growth promotion. Inoculation of seeds, grown in the presence of copper or creosote. with either native or transformed P. asplenii AC significantly increased root and shoot biomass. Native and transformed P. asplenii AC and transformed P asplenii AC encapsulated in alginate were equally effective at promoting plant growth in copper-contaminated soils. In creosote-contaminated soils the native bacterium was the least effective, and the transformed encapsulated bacterium was the most effective in growth promotion.
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