期刊
RESEARCH IN MICROBIOLOGY
卷 156, 期 10, 页码 1031-1037出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.resmic.2005.05.009
关键词
Halanaerobium; rhodanese; thiosulfate reducers
类别
The recently described anaerobic moderately halophilic bacterium Halanaerobium congolense has been shown to reduce thiosulfate and sulfur-but not sulfate-into sulfide. When cultivated in the presence of thiosulfate as terminal electron acceptor, H. congolense possesses a highly active thiosulfate:cyanide sulfur-transferase activity (rhodanese-like enzyme). A gene library of H. congolense (DSM I 1287(T)) was constructed, and a 3.1-kb Sau3A DNA that encompassed a thiosulfate:cyanide sulfur-transferase-encoding gene was isolated in Escherichia coli. This fragment contains 2 orfs, which were separately subcloned in E. coli. The 900-bp gene encoding the rhodanese-like protein was named rdlA. RdIA differs from other known rhodanese-like proteins by having two potential catalytic sites, one N-terminal and one C-terminal, both harboring a cysteine. The two putative active sites are preceded by a highly-conserved region of unknown function. Closely related genes were also characterized in other thiosulfate-reducing non-sulfate-reducing anaerobes belonging to phylogenetically distant microorganisms, thus suggesting that RdIA is of importance in the mechanism of thiosulfate reduction by numerous members of the domain Bacteria. (C) 2005 Elsevier SAS. All rights reserved.
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