期刊
JOURNAL OF CELL SCIENCE
卷 118, 期 23, 页码 5499-5511出版社
COMPANY BIOLOGISTS LTD
DOI: 10.1242/jcs.02667
关键词
trypanosome; glycosylphosphatidylinositol; flagellar pocket; transferrin receptor; protein sorting
类别
资金
- NIAID NIH HHS [AI35739, R01 AI035739] Funding Source: Medline
Progression of GPI-anchored proteins in bloodstream African trypanosomes correlates with GPI-valence: homodimeric VSG (2 GPI) is a surface protein; heterodimeric transferrin receptor (1 GPI) localizes in the flagellar pocket; homodimeric GPI-minus VSG (0 GPI) is rapidly degraded in the lysosome. We test this relationship using three native secretory/endocytic proteins as monomeric GPI-plus and -minus reporters. GPI-minus procyclin trafficks to the lysosome and is degraded. GPI-plus procyclin trafficks to the flagellar pocket/cell surface and is released (similar to 50%) with an intact anchor, the remainder (similar to 50%) is degraded in the lysosome. GPI-plus BiPNHP, derived from the ER marker BiP, is released quantitatively (> 80%), while GPI-plus p67HP, derived from the lysosomal marker p67, turns over by both release (similar to 15%) and lysosomal degradation (> 50%). Turnover of endogenous transferrin receptor occurs primarily by lysosomal degradation (> 90%). Thus shedding of monovalent GPI reporters correlates inversely with lysosomal targeting. We propose that mono-GPI reporters cycle through the flagellar pocket and endosome until they are disposed of by either shedding or lysosomal targeting. Partitioning between these fates may be a function of individual physical properties. Release is likely due to the exclusive use of C-14:0 myristate in the bloodstream stage GPI anchor. Up-regulation of transferrin receptor by culture in dog serum resulted in prominent cell surface localization, but not in elevated release. Surface receptor was non-functional for ligand binding suggesting that it may be bivalent homodimers of the GPI-anchored ESAG6 receptor subunit.
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