期刊
PLANT PHYSIOLOGY AND BIOCHEMISTRY
卷 43, 期 12, 页码 1052-1060出版社
ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
DOI: 10.1016/j.plaphy.2005.10.006
关键词
l-deoxy-D-xylulose 5-phosphate synthase; Lycopersicon esculentum; phytochrome; phytoene synthase; ripening; tomato
The accumulation of carotenoids greatly influences the quality of ripe tomato (Solanum lycopersicum) fruit because these compounds contribute to their color and functional food value. The regulatory properties and gene expression of phytoene synthase (PSY), a key enzyme involved in carotenoid biosynthesis, was analyzed in tomato fruit. PSY activity was detected in the soluble fraction showing a time- and a protein-dependent increase in the formation of phytoene from geranylgeranyl pyrophosphate (GGPP). The enzyme was stimulated by Mg2+ and ATP, and was inhibited by Tween 20 and Triton X-100. The enzyme followed typical Michaelis-Menten kinetics with increasing GGPP. During in vivo studies, pericarp discs from breaker stage tomatoes were ripened in darkness (D), or D interrupted by daily pulses of red (R) light, or R followed by far red (FR) light. After 14 days incubation, R-treated discs had accumulated 12 mg carotenoids/100 g fresh weight; nearly a 50% increase over D- and R/FR-treated discs. This R/FR reversibility of carotenoid accumulation was also observed in PSY activity 8 days post breaker, where it showed peak activity. However, this R/FR regulation of PSY activity was not reflected in PSY1 transcript levels, suggesting that PSY may be subject to post-translational regulation. (c) 2006 Published by Elsevier SAS.
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