期刊
GROWTH FACTORS
卷 23, 期 4, 页码 313-321出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/08977190500252763
关键词
BMP2; cartilage; differentiation; mesenchymal; stem cells; TGF beta 1
This study investigated the effects of TGF beta 1, BMP2 or a combination of both on the chondrogenic differentiation of mesenchymal stem cells (MSCs) in a serum-free micromass culture system in vitro . Putative MSCs harvested from the iliac crest of 4-5 month old New Zealand White Rabbits were expanded and cultured in three-dimensional high density micromass aggregate cultures containing TGF beta 1, BMP2 or a combination of both, in the absence of serum. After 14-20 days of culture, chondrogenic differentiation of the MSCs was assayed by toluidine blue staining, immunohistochemistry and semi-quantitative RT-RCR of type I collagen (CI) and type II collagen (CII). Quantitative measurements of cell proliferation and sulfated glycosaminoglycan (s-GAG) were also carried out to assess the growth rate and matrix deposition of the cultured aggregates. Both immunohistochemical staining and semi-quantitative RT-PCR showed that the combination of BMP2 and TGF beta 1 resulted in a marked enhancement of collagen II synthesis, with minimal collagen I expression, which would suggest hyaline-like cartilage formation. Additionally, BMP2 and TGF beta 1 had a synergistic effect on matrix proteoglycan deposition, as assessed by metachromatic toluidine blue staining. This is consistent with the quantitative measurement of glycosaminoglycans, whereby a significant increase in GAG/DNA was noted in the co-treatment group. Hence, it can be concluded that the combination of BMP2 and TGF beta 1 has a synergistic effect on the differentiation of MSC into hyaline-like cartilage tissue.
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