期刊
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE
卷 16, 期 12, 页码 1131-1136出版社
SPRINGER
DOI: 10.1007/s10856-005-4718-3
关键词
-
Phosphate-based fibres of the generic composition (CaO)(0.46)-(Na2O)(n)-(Fe2O3)(y)-(P2O5)(0.50) have been evaluated, in vitro, as three dimensional scaffolds for tissue engineering of the hard-soft tissue interface by assessing the fibre solubility and growth and functional gene expression of human cells. Primary human osteoblasts and fibroblasts were seeded onto scaffolds and maintained in culture for up to 21 days. Fluorescent immunolabeling revealed the spread cell morphology and significant proliferation pattern on these fibres, particularly on the 3 mol% Fe2O3-containing formulation. Real-time quantitative Polymerase Chain Reaction (rtQ-PCR) analysis of gene expression using TaqMan(R) Probes was preformed and it has been established that committed cell differentiation was maintained by both cell types, and was strongly related to the 3 mol% Fe2O3 glass composition. These novel, readily manufactured, soluble glass fibres offer a biocompatible and biochemically favourable alternative in the search for suitable degradable materials used in Tissue Engineering. (C) 2005 Springer Science + Business Media, Inc.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据