期刊
DEVELOPMENT
卷 132, 期 23, 页码 5271-5281出版社
COMPANY BIOLOGISTS LTD
DOI: 10.1242/dev.02138
关键词
Ultrabithorax; Drosophila; Hox; transcriptional activation; transcriptional repression; sex combs reduced
资金
- NICHD NIH HHS [HD28315] Funding Source: Medline
While testing the functions of deletion mutants in the Hox protein Ultrabithorax (Ubx), we found that the embryonic repression function of Ubx on Distal-less transcription in limb primordia is highly concentration dependent. The steep sigmoidal relationship between in vivo Ubx concentration and Distal-less repression is dependent on the Ubx YPWM motif. This suggests that Ubx cooperatively assembles a multi-protein repression complex on Distal-less regulatory DNA with the YPWM motif as a key protein-protein interface in this complex. Our deletion mutants also provide evidence for a transcriptional activation domain in the N-terminal 19 amino acids of Ubx. This proposed activation domain contains a variant of the SSYF motif that is found at the N termini of many Hox proteins, and is conserved in the activation domain of another Hox protein, Sex combs reduced. These results suggest that the N-terminal region containing the SSYF motif has been conserved in many Hox proteins for its role in transcriptional activation.
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