期刊
FOOD CHEMISTRY
卷 93, 期 4, 页码 591-597出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2004.10.042
关键词
saponin; isoflavonoid; soybean glycoside; medicinal foodstuffs
We studied the chemical characteristics of soyasaponin P g with a view to establishing an effective fractionating method for soybean glycoside. Under an acidic condition of below pH 3, soyasaponin beta g precipitated with unknown sugar and glycoside compounds of soybean. In addition, soyasaponin beta g was hydrolyzed to soyasaponin Bb and 2,3-dihydro-2,5-dihydroxy-6-methyl-4H-pyran-4-one moieties under alkaline conditions. 1.0 mM NaOH hydrolyzed soyasaponin beta g at the rate of 0.44 mu mol/min. Metal ion-binding activity of soyasaponin beta g resulted in 2,3-dihydro-2,5-dihydroxy-6-methyl-4H-pyran-4-one moiety and I mM soyasaponin beta g chelated with 1.57 mM Fe2+ and 0.2 mM Cu2+. Based on these chemical characteristics of soyasaponin P g, we fractionated soybean saponin from soy-waste after oil extraction (industrial waste) by four steps: (1) precipitation of glycosides under acidic conditions (pH 2). (2) separation of hydrophobic functional compound (isoflavonoid), (3) chelate precipitation of 2,3-dihydro-2,5-dihydroxy-6-methyl-4H-pyran-4-one conjugated saponin using FeCl2 and (4) removal of the 2,3-dhydro-2,5-dihydroxy-6-methyl-4H-pyran-4-one and Fe2+ complex by an alkaline hydrolysis (pH 12). Finally, 375 mg of group B saponin (84% purity) were obtained from 100 g of soy-waste after oil extraction. This fractionating method is a simple and useful method for producing medicinal foodstuffs from soybean. (c) 2004 Elsevier Ltd. All rights reserved.
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