Identification of leukemia-associated antigens in chronic myeloid leukemia by proteomic analysis

The immune system plays ail important role in the treatment of chronic myeloid leukemia (CML). Identification of leukemia-associated antigens (LAAs) eliciting ail immune response in patients is a prerequisite for specific immunotherapy of CML. To identify new LAAs in CML NVe utilized a novel approach based serology and protcomics technologies. LAAs were identified by comparing the reactivity of proteins resolved by 2-DE with sera front CML patients and healthy donors. Several new LAAs were identified including alpha enolase, aldolase A. HSP70 protein8, beta-tubulin and tropomyosin isoforms. Although, the functions of these identified proteins in CML need further investigation, the detection of autoantibodies in CML may have value on CML screening, diagnosis, or follow-up. Additionally, identification of LAAs in CML may also be of vital importance in anti-en-based immunotherapy. (C) 2005 Elsevier Ltd. All rights reserved.