期刊
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
卷 69, 期 12, 页码 2466-2469出版社
TAYLOR & FRANCIS LTD
DOI: 10.1271/bbb.69.2466
关键词
Agaricus blazei (A. blazei); natural killer (NK); interferon (IFN)-gamma
Aqueous extracts of the Agaricus blazei fruiting body prepared at different temperatures were fractionated by ethanol precipitation with various ethanol concentrations. The original aqueous extracts of A. blazei failed to stimulate natural killer (NK) cell activity in murine spleen cells in vitro, but the strongest effect was observed in a 30% ethanol-soluble-50% ethanol-insoluble fraction prepared from the extract at 40 degrees C (fraction A-50). Fraction A-50 also showed the strongest augmenting effect on interferon (IFN)-gamma production. This augmentation of NK activity and IFN-gamma production by fraction A-50 was completely abrogated by a heat treatment.
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