期刊
JOURNAL OF CELL BIOLOGY
卷 171, 期 5, 页码 845-855出版社
ROCKEFELLER UNIV PRESS
DOI: 10.1083/jcb.200505071
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- NCI NIH HHS [5T32CA09151, T32 CA009151] Funding Source: Medline
- NIGMS NIH HHS [R01 GM035527, R37 GM035527, GM35527] Funding Source: Medline
Mechanisms underlying the organization of centrosome-derived microtubule arrays are well understood, but less is known about how acentrosomal microtubule networks are formed. The basal cortex of polarized epithelial cells contains a microtubule network of mixed polarity. We examined how this network is organized by imaging microtubule dynamics in acentrosomal basal cytoplasts derived from these cells. We show that the steady-state microtubule network appears to form by a combination of microtubule-microtubule and microtubule-cortex interactions, both of which increase microtubule stability. We used computational modeling to determine whether these microtubule parameters are sufficient to generate a steady- state acentrosomal microtubule network. Microtubules undergoing dynamic instability without any stabilization points continuously remodel their organization without reaching a steady- state network. However, the addition of increased microtubule stabilization at microtubule-microtubule and microtubule-cortex interactions results in the rapid assembly of a steady-state microtubule network in silico that is remarkably similar to networks formed in situ. These results define minimal parameters for the self-organization of an acentrosomal microtubule network.
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