期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 102, 期 49, 页码 17693-17698出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.0506687102
关键词
chromatin immunoprecipitation; genomics; stringent response; transcription
资金
- Wellcome Trust Funding Source: Medline
Chromatin immunoprecipitation and high-density microarrays have been used to monitor the distribution of the global transcription regulator Escherichia coli cAMP-receptor protein (CRP) and RNA polymerase along the E. coli chromosome. Our results identify targets occupied by CRP and genes transcribed by RNA polymerase in vivo. Many of the loci of CRIP binding are at known CRIP regulated promoters. However, our results show that CRP also interacts with thousands of weaker sites across the whole chromosome and that this background binding can be used as a probe for organization within the E. coli folded chromosome. In rapidly growing cells, we show that the major sites of RNA polymerase binding are approximate to 90 transcription units that include genes needed for protein synthesis. Upon the addition of rifampicin, RNA polymerase is distributed among >500 functional promoters. We show that the chromatin immunoprecipitation and high-density-microarrays methodology can be used to study the redistribution of RNA polymerase induced by environmental stress, revealing previously uncharacterized aspects of RNA polymerase behavior and providing an alternative to the transcriptomics approach for studying global transcription patterns.
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