期刊
TETRAHEDRON LETTERS
卷 46, 期 50, 页码 8727-8731出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.tetlet.2005.10.048
关键词
Croton stellatopilosus; Euphorbiaceae; terpenoid biosynthesis; chloroplast; geranylgeranyl diphosphate phosphatase; geranylgeraniol
The process of catalytic dephosphorylation of geranylgeranyl diphosphate (GGPP) to give geranylgeraniol (GGOH) in Croton stellatopilosus leaves was examined by in vivo chloroplast feedings with [1-H-3]GGPP and [1-H-3]GGMP and in vitro enzyme-catalyzed reactions. The results strongly suggest that the formation of GGOH from GGPP proceeds in the chloroplasts via two successive monodephosphorylation reactions. Hence, we name the enzyme geranylgeranyl diphosphate phosphatase rather than geranylgeranyl diphosphatase based on its catalytic mechanism. (c) 2005 Elsevier Ltd. All rights reserved.
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