期刊
GENES & DEVELOPMENT
卷 19, 期 24, 页码 2969-2978出版社
COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT
DOI: 10.1101/gad.1362305
关键词
CPF complex; gene loops; RNA polymerase II; Ssu72; transcription termination
资金
- NIGMS NIH HHS [R01 GM039484, GM-39484, R01 GM068887, GM-6888] Funding Source: Medline
The prevailing view of the RNA polymerase 11 (RNAP 11) transcription cycle is that RNAP 11 is recruited to the promoter, transcribes a linear DNA template, then terminates transcription and dissociates from the template. Subsequent rounds of transcription are thought to require de novo recruitment of RNAP 11 to the promoter. Several recent findings, including physical interaction of T-end processing factors with both promoter and terminator regions, challenge this concept. Here we report a physical association of promoter and terminator regions of the yeast BUD3 and SEN1 genes. These interactions are transcription-dependent, require the Ssu72 and Pta1 components of the CPF 3'-end processing complex, and require the phosphatase activity of Ssu72. We propose a model for RNAP 11 transcription in which promoter and terminator regions are juxtaposed, and that the resulting gene loops facilitate transcription reinitiation by the same molecule of RNAP 11 in a manner dependent upon Ssu72-mediated CTD dephosphorylation.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据