Selection of 5′-untranslated sequences that enhance initiation of translation in a cell-free protein synthesis system from wheat embryos

Random libraries of mRNA 5'-leader sequences were screened to obtain some sequences that can stimulate the translation initiation in a cell-free translation system from wheat embryos as efficiently as the Q sequence from tobacco mosaic virus. Several sequences that are as useful as the Q sequence and are homologous to no known sequences survived the screening. We expect that these sequences add useful options to the cell-free protein synthesis system that is becoming a powerful tool in the post-genomic researches. (c) 2005 Elsevier Ltd. All rights reserved.