期刊
GENES & DEVELOPMENT
卷 19, 期 24, 页码 2947-2952出版社
COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT
DOI: 10.1101/gad.1372505
关键词
microRNA; iab-4; Ultrabithorax; homeotic gene
资金
- NIGMS NIH HHS [F32 GM072395, R01 GM034431, GM34431, GM72395] Funding Source: Medline
The Drosophila Bithorax Complex encodes three wellcharacterized homeodomain proteins that direct segment identity, as well as several noncoding RNAs of unknown function. Here, we analyze the iab-4 locus, which produces the microRNAs iab-4-5p and iab-4-3p. iab-4 is analogous to miR-196 in vertebrate Hox clusters. Previous studies demonstrate that miR-196 interacts with the Hoxb8 3' untranslated region. Evidence is presented that miR-iab-4-5p directly inhibits Ubx activity in vivo. Ectopic expression of mir-iab-4-5p attenuates endogenous Ubx protein accumulation and induces a classical homeotic mutant phenotype: the transformation of halteres into wings. These findings provide the first evidence for a noncoding homeotic gene and raise the possibility that (other such genes occur within the Bithorax complex. We also discuss the regulation of mir-iab-4 expression during development.
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