期刊
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
卷 830, 期 1, 页码 100-104出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jchromb.2005.10.040
关键词
ginseng; ginsenoside Re; chromatographic immunostaining; NIH Image software
A chromatographic immunostaining method has been developed for the determination of ginsenoside Re (G-Re) in ginseng samples on a polyethersulphone (PES) membrane. G-Re standard and the extracts of ginseng roots were applied to a PES membrane and developed by methanol-water-acetic acid (45:55: 1, by volume). G-Re was clearly detected by an immunostaining method using a monoclonal antibody against G-Re. The coloring spots of G-Re were analyzed quantitatively using NIH Image software indicating at least 0.125 mu g of G-Re was detectable. G-Re can be analyzed quantitatively between 0.25 and 40 mu g. (c) 2005 Published by Elsevier B.V.
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