期刊
JOURNAL OF NEUROSCIENCE
卷 26, 期 1, 页码 345-353出版社
SOC NEUROSCIENCE
DOI: 10.1523/JNEUROSCI.2755-05.2006
关键词
brain development; microarray; cortex; hippocampus; hypothalamus; developmental disorders
资金
- NIDA NIH HHS [R01 DA 13386, R01 DA013386] Funding Source: Medline
- NIMH NIH HHS [P01 MH042251, P01 MH42251] Funding Source: Medline
Neural development involves the expression of ensembles of regulatory genes that control the coordinate and region- specific expression of a host of other genes, resulting in the unique structure, connectivity, and function of each brain region. Although the role of some specific genes in neural development has been studied in detail, we have no global view of the orchestration of spatial and temporal aspects of gene expression across multiple regions of the developing brain. To this end, we used transcriptional profiling to examine expression levels of 9955 genes in the hypothalamus, hippocampus, and frontal cortex across seven stages of postnatal development and up to four stages of prenatal development in individual male rats (six per group). The results reveal dramatic changes across development in > 97% of the neurally expressed genes. They also uncover a surprising degree of regional differentiation occurring after birth and through the first 2 weeks of life. Cluster analysis identifies 20 clusters of transcripts enriched in genes related to particular functions, such as DNA metabolism, nuclear function, synaptic vesicle transport, myelination, and neuropeptide hormone activity. Thus, groups of genes with related functions change in the brain at specific times, possibly marking critical periods for each function. These findings can broadly serve as a backdrop for studying the role of individual genes in neural development. They also underscore the importance of early postnatal life in the rat, which corresponds to late gestation in the human, as a critical late phase of neural organization and differentiation, even in subcortical regions.
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