期刊
BIOTECHNOLOGY AND BIOENGINEERING
卷 93, 期 1, 页码 48-55出版社
WILEY
DOI: 10.1002/bit.20690
关键词
aldolases; stereoselective synthesis; DHAP; secondary reactions
The effect of secondary reactions on DHAP-dependent aldolase stereoselective synthesis yields is reported. The fuculose-1-phosphate aldolase catalyzed synthesis between DHAP and Cbz-S-alaninal has been chosen as case study. It has been demonstrated that DHAP is not only chemically degraded in the reaction medium, but also enzymatically. The last reaction has been shown to take place when type II aldolases are used as biocatalysts. In order to minimize the effect of non-desired reactions, temperature reduction has been shown to be favorable, and operation at 4 degrees C has been chosen as appropriate. On the other hand, the fed-batch addition of DHAP also increased the synthesis yields and, combined with low temperature, led to almost quantitative conversion. (c) 2005 Wiley Periodicals, Inc.
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