Determination of L-ascorbic acid in wines by direct injection liquid chromatography using a polymeric column

A rapid method for the identification and quantification of L-ascorbic acid in wines by direct injection liquid chromatography equipped with a UV detection was developed. The levels of ascorbic acid were determined using a polymeric PLRP-S 100 A (5 mu m) column (150 mm x 4.6 mm) with a mobile water/trifluoroacetic acid (99/1, v/v) phase. The method is rapid (less than 5 min) and sensitive (LOQ of 5 mg L(-1)). The calibration curve of ascorbic acid was linear (r = 0.999) over a concentration range between 1 and 200 mg L(-1). Repeatability was less than 2.5% and the recovery over 95%. (c) 2005 Elsevier B.V. All rights reserved.