Simple and sensitive determination of free and total morphine in human liver and kidney using gas chromatography-mass spectrometry

We developed a reliable, simple and sensitive method to determine free and total morphine in human liver and kidney, using gas chromatography-mass spectrometry (GC-MS). Free morphine or total morphine obtained by acid hydrolysis from 0.2 g tissue sample was extracted using an Extrelut((R)) NT column with an internal standard, dihydrocodeine, followed by trimethylsilylation. The derivatized extract was submitted to GC-MS analysis of EI-SIM mode. The calibration curves of morphine in both liver and kidney samples were linear in the concentration range from 0.005 to 5 mu g/g. The lower limits of detection of morphine were 0.005 mu g/g. This method proved successful when we determined free and total morphine in liver and kidney obtained from an autopsied man who was mis-ingested morphine compound in the hospital, which resulted in the cause of death being morphine intoxication. (c) 2005 Elsevier B.V. All rights reserved.