期刊
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
卷 128, 期 2, 页码 473-484出版社
AMER CHEMICAL SOC
DOI: 10.1021/ja056328f
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Density functional calculations were performed in response to the controversies regarding the identity of the oxidant species in cytochrome P450. The calculations were used to gauge the relative C-H hydroxylation reactivity of three potential oxidant species of the enzyme, the high-valent oxo-iron species Compound I (Cpd I), the ferric hydroperoxide Compound 0 (Cpd 0), and the ferric-hydrogen peroxide complex Fe(H2O2). The results for the hydroxylation of a radical probe substrate, 1, show the following trends: (a) Cpd I is the most reactive species; in its presence the other two reagents will be silent. (b) In the absence of Cpd 1, substrate oxidation by Cpd 0 and Fe(H2O2) will take place via a stepwise mechanism that involves initial O-O homolysis followed by H-abstraction from 1. (c) Cpd 0 will undergo mostly porphyrin hydroxylation and only similar to 15% of substrate oxidation producing mostly the rearranged alcohol, 3 (Scheme 2). (d) Fe(H2O2) will generate mostly free hydrogen peroxide (uncoupling). A small fraction will perform substrate oxidation and lead mostly to 3. Reactivity probes for these reagents are kinetic isotope effect (KIE) and the product ratio of unrearranged to rearranged alcohols, [2/3]. Thus, for substrate oxidation by Cpd 0 or Fe(H2O2) KIE will be small, similar to 2, while Clod I will have large KIE values. Typically both Cpd 0 and Fe(H2O2) Will lead to a (2/3) ratio < 1, while Cpd I will lead to ratios > 1. In addition, the product isotope effect (KIE2/KIE3 not equal 1) is expected from the reactivity of Cpd 1.
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