期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1103, 期 1, 页码 118-124出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2005.11.007
关键词
sugarcane (Saccharum officinarum L. Gramineae); flavonoids; HPLC-UV; transgenic plants
A high-performance liquid chromatography (HPLC) method with photo-diode array (DAD) detection was developed to separate and quantify flavonoids in sugarcane leaves and bagasse (= the crushed sugarcane refuse from juice extraction), and in sugarcane juice. Sugarcane flavonoids consist of a complex mixture of aglycones and glycosides (including flavonolignan glycosides), and the HPLC-UV method herein proposed is suitable for their quantification as total flavonoids. This method was applied to analyze samples of cultivated sugarcane, commercial juice and transgenic sugarcane leaves. Sugarcane leaves proved a promising source of flavonoids: an average of 1.10 mg of total flavonoids/g plant material was found in fresh leaves. Moreover, the flavonoid content of sugarcane juice (0.6 mg/mL) is comparable to other food Sources of flavonoids previously reported. Transgenic sugarcane leaves (Bowman-Birk and Kunitz) were compared with non-modified (control) plant samples using the proposed HPLC-UV method, which indicated that the content of total flavonoids in transgenic plants is different from that in non-modified sugarcane. (c) 2005 Elsevier B.V. All rights reserved.
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