期刊
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
卷 40, 期 1, 页码 157-161出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jpba.2005.04.003
关键词
microemulsion electrokinetic chromatography; Andrographis paniculata; andrographolide; dehydroandrographolide
The present paper describes the development of a microemulsion electrokinetic chromatographic (MEEKC) method for simultaneous determination of andrographolide and dehydroandrographolide in traditional Chinese medicines and Chinese medicinal preparations. The MEEKC method involved the use of sodium dodecyl sulfate (SDS) as surfactant, heptane as organic solvent and butan-1-ol as co-solvent. The effect of temperature and pH of running buffers on separation were examined. The optimized conditions (heptane 0.81 % (w/w), SDS 3.31% (w/w), butan-1-ol 6.61% (w/w) and 10 mM sodium tetraborate buffer, pH 9.2) allowed a useful and good reproducible separation of the studied analytes to be achieved. (c) 2005 Elsevier B.V. All rights reserved.
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