期刊
JOURNAL OF EXPERIMENTAL MEDICINE
卷 203, 期 1, 页码 177-188出版社
ROCKEFELLER UNIV PRESS
DOI: 10.1084/jem.20052288
关键词
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资金
- Intramural NIH HHS Funding Source: Medline
Uptake of Leishmania major by dendritic cells (DCs) results in activation and interleukin (IL)-12 release. Infected DCs efficiently stimulate CD4(+) and CD8(+) T cells and vaccinate against leishmaniasis. In contrast, complement receptor 3-dependent phagocytosis of L. major by macrophages (M Phi) leads exclusively to MHC class II-restricted antigen presentation to primed, but not naive, T cells, and no IL-12 production. Herein, we demonstrate that uptake of L. major by DCs required parasite-reactive immunoglobulin (Ig) G and involved Fc gamma RI and Fc gamma RIII. In vivo, DC infiltration of L. major-infected skin lesions coincided with the appearance of antibodies in sera. Skin of infected B cell-deficient mice and Fc gamma(-/-) mice contained fewer parasite-infected DCs in vivo. Infected B cell-deficient mice as well as Fc gamma(-/-) mice (all on the C57BL/6 background) showed similarly increased disease susceptibility as assessed by lesion volumes and parasite burdens. The B cell-deficient mice displayed impaired T cell priming and dramatically reduced IFN gamma production, and these deficits were normalized by infection with IgG-opsonized parasites. These data demonstrate that DC and M Phi use different receptors to recognize and ingest L. major with different outcomes, and indicate that B cell-derived, parasite-reactive IgG and DC Fc gamma RI and Fc gamma RIII are essential for optimal development of protective immunity.
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