期刊
YEAST
卷 23, 期 3, 页码 173-183出版社
WILEY
DOI: 10.1002/yea.1347
关键词
genetics; microscopy; Schizosaccharomyces pombe; Synchrony; transformation
资金
- NIGMS NIH HHS [GM-059321, GM-069957, R01 GM069957, R01 GM059321, R01 GM069957-03] Funding Source: Medline
The fission yeast Schizosaccharomyces pombe is a popular model system, and has been particularly influential in studies of the cell cycle and chromosome dynamics. Despite its differences from Saccharomyces cerevisiae, the tools and methods for fission yeast are conceptually similar to those used in budding yeast. Here, we present basic methods sufficient for a beginner in this system to carry out most required manipulations for genetic analysis or molecular biology. Copyright (C) 2006 John Wiley & Sons, Ltd.
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