期刊
JOURNAL OF BACTERIOLOGY
卷 188, 期 3, 页码 1180-1183出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/JB.188.3.1180-1183.2006
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资金
- NIAID NIH HHS [R43 AI051104, AI51104, R44 AI051104] Funding Source: Medline
An Escherichia coli mutant was isolated and shown to be polymyxin B resistant. Mapping and sequence analysis revealed a missense mutation at codon 53 within the pmrA (basR) gene that results in a G-to-V substitution. Fusions of promoters from the pmrC, yibD, and pmrH genes with the lacZ reporter showed that they were constitutively expressed in pmrA53 cells. In pmrA(+) strains, these promoters were induced by iron and zinc, while a Delta pmrA mutation blocked induction. The PmrA regulon regulates genes whose products remodel the composition and charge of lipid A and hence the barrier properties of the outer membrane. Along these lines, the pmrA53 mutant was also found to be hypersensitive to the anionic bile detergent deoxycholic acid.
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