期刊
VIRUS RESEARCH
卷 115, 期 2, 页码 131-140出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.virusres.2005.07.013
关键词
Japanese encephalitis virus; flavivirus; polypyrimidine tract-binding protein (PTB); gel mobility shift assay; UV-induced cross-linking assay; immunoprecipitation
类别
The 3' stem-loop (SL) region of positive- and negative-strand RNA of Japanese encephalitis virus (JEV), like that of other flaviviruses, may function as cis-acting signals during RNA replication. In order to demonstrate the specific interaction between JEV 3' SL regions and BHK-21 cellular proteins, we performed gel mobility shift assay and UV-induced cross-linking assay. We identified seven cellular proteins of 110, 87, 67, 45, 38, 34, and 30 kDa that bound to the (+)3' SL RNA, and eight cellular proteins of 138, 110, 87, 67, 55, 52, 38, and 34 kDa that bound to the (-)3' SL RNA. The 55 kDa protein was identified as the polypyrimidine tract-binding (PTB) protein by immunoprecipitation assay. These data suggest that the 3' SL regions of JEV-RNA of both polarities may act as recruiting signals for the components of viral replication complexes including host cell-derived PTB protein. (c) 2005 Elsevier B.V. All rights reserved.
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