Microbacterium koreense sp nov, from sea water in the South Sea of Korea

Microbacterium, strains JS53-2(T) and JS53-5 were isolated from sea water in the South Sea of Korea and subjected to phenotypic, chemotaxonomic and genetic characterization. The cells were found to be Gram-positive. These strains contained MK-11 and MK-12 as the main respiratory quinones and anteiso-C-15:0, anteiso-C-17:0 and iso-C-16:0 as the major cellular fatty acids. The DNA G + C content was 68 mol%. The cell-wall sugars of the isolates were galactose and xylose, and the diamino acid in the cell-wall hydrolysates was lysine. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the isolates represented an evolutionary lineage distinct from those of other Microbacterium species. DNA-DNA reassociation values between the isolates and reference strains Microbacterium, terregens KCTC 19034, Microbacterium, lacticum, KCTC 9230(T), Microbacterium aurum KCTC 19091(T) and Microbacterium schleiferi KCTC 19095(T) were below 23%. On the basis of evaluation of the morphological, physiological and chemotaxonomic characteristics, 16S rRNA gene sequence comparisons and DNA-DNA hybridizations, a novel species, Microbacterium koreense sp. nov., is proposed for these isolates. The type strain is JS53-2(T) (=KCTC 19074(T)=CIP 108696(T) =CCUG 50754(T)).