期刊
BIOLOGICAL & PHARMACEUTICAL BULLETIN
卷 29, 期 2, 页码 211-215出版社
PHARMACEUTICAL SOC JAPAN
DOI: 10.1248/bpb.29.211
关键词
Saururus chinensis; cyclooxygenase-2 5-lipoxygenase; mast cell; asthma; cosinophil
As an attempt to find bioactive medicinal herbs exerting anti-asthmatic activity, the effects of an ethanol extract from the parts of Saururus chinensis were evaluated in both in vitro and in vivo. The ethanol extract of S. chinensis (ESC) inhibited generation of the cyclooxygenase-2 (COX-2) dependent phases of prostaglandin D-2 in bone marrow-derived mast cells in a concentration-dependent manner with an IC50 value of 14.3 mu g/ml. ESC also inhibited leukotriene C-4 production with an IC50 value of 0.3 mu g/ml. This demonstrates that ESC has COX-2/5-lipoxygenase dual inhibitory activity. In addition, this compound inhibited degranulation reaction in a dose dependent manner, with an IC50 value of 1.3 mu g/ml. An ovalbumin induced mouse asthmatic animal model was used to determine its in vivo anti-asthmatic activity. The oral administration (50-200 mg/kg) of ESC reduced the number of infiltrated eosinophil in a bronchoalveolar lavage fluid. Furthermore, ESC (100 mg/kg) inhibited the eotaxin and IL-4 mRNA expression levels. These results suggest that the anti-asthmatic activity of S. chinensis might in part occur via the inhibition of eicosanoid generation, degranulation as well as the down regulation of IL-4 and eotaxin mRNA expression.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据