期刊
NATURE BIOTECHNOLOGY
卷 24, 期 2, 页码 185-187出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/nbt1177
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资金
- NCRR NIH HHS [R24-RR017721] Funding Source: Medline
- PHS HHS [P20-GMO69981] Funding Source: Medline
We have previously reported that high concentrations of basic fibroblast growth factor (bFGF) support feeder-independent growth of human embryonic stem (ES) cells, but those conditions included poorly defined serum and matrix components. Here we report feeder-independent human ES cell culture that includes protein components solely derived from recombinant sources or purified from human material. We describe the derivation of two new human ES cell lines in these defined culture conditions.
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