期刊
FEMS MICROBIOLOGY LETTERS
卷 255, 期 1, 页码 115-120出版社
BLACKWELL PUBLISHING
DOI: 10.1111/j.1574-6968.2005.00072.x
关键词
Escherichia coli; horizontal gene transfer; biofilm; colony; plasmid; transformation
类别
We tested the possibility of nonconjugative lateral DNA transfer in a colony biofilm of mixed Escherichia coli strains. By simply coculturing a plasmid-free F- strain and another F- strain harboring a nonconjugative plasmid in a colony biofilm on antibiotic-free agar media, transformed cells were produced within 24-48 h at the frequency of 10(-10)-10(-9) per recipient cell. PCR analysis of the transformed cells demonstrated the occurrence of lateral plasmid transfer. These cells survived until at least day 7 under antibiotic-free conditions. Liquid cultures of the same strains in Luria-Bertani broth produced no or few transformants, suggesting the importance of colony-biofilm formation for plasmid transfer. This is a novel line of evidence indicating that nonconjugative, nonviral horizontal gene transfer can occur between E. coli cells.
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