期刊
CANADIAN JOURNAL OF FOREST RESEARCH
卷 36, 期 2, 页码 337-350出版社
CANADIAN SCIENCE PUBLISHING
DOI: 10.1139/X05-276
关键词
-
类别
Reliable and rapid identification of exotic pest species is critical to biosecurity. However, identification of morphologically indistinct specimens, such as immature life stages, that are frequently intercepted at borders is often impossible. Several DNA-based methods have been used for species identification; however, a more universal and anticipatory identification system is needed. Consequently, we tested the ability of DNA barcodes to identify species of tussock moths (Lymantriidae), it family containing several important pest species. We sequenced a 617 base pair fragment of the mitochondrial gene cytochrome c oxidase 1 for 20 lymantriid species. We used these, together with other Noctuoidea species sequences from GenBank and the Barcoding of Life Database to create it profile or reference sequence data set. We then tested the ability of this profile to provide correct species identifications for 93 additional lymantriid specimens from it data set of mock unknowns. Of the unknowns, 100% were correctly identified by the cytochrome c oxidase 1 profile. Mean interspecific sequence (Kimura 2-parameter) divergence was all order of magnitude greater (14%) than mean intraspecific divergence (< 1%). Four species showed deeper genetic divergences among populations. We conclude that DNA barcodes provide it highly accurate means of identifying lymantriid species and show considerable promise as a Universal approach to DNA-based identification of pest insects.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据