4.5 Article

High-affinity, slowly desensitizing GABAA receptors mediate tonic inhibition in hippocampal dentate granule cells

期刊

MOLECULAR PHARMACOLOGY
卷 69, 期 2, 页码 564-575

出版社

AMER SOC PHARMACOLOGY EXPERIMENTAL THERAPEUTICS
DOI: 10.1124/mol.105.016683

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资金

  1. NINDS NIH HHS [R01-NS040337, R01-NS044370, R01 NS044370-04, R01 NS040337-03, R01 NS044370, R01 NS040337] Funding Source: Medline

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The tonic form of GABA-mediated inhibition requires the presence of slowly desensitizing GABA A receptors with high affinity, which has not yet been directly demonstrated in hippocampal neurons. Low concentration of GABA (1 mu M) persistently increased baseline noise, increased membrane slope conductance, but did not affect spontaneous inhibitory postsynaptic currents (sIPSCs) in dentate granule cells (DGCs). Higher concentrations of GABA (10-100 mu M) desensitized synaptic currents quickly, and there was a large residual current. Saturating concentration of GABA (1 mM) completely desensitized synaptic currents and revealed a slowly desensitizing, persistent current. Penicillin ( 300 mu M) inhibited baseline noise without affecting mean current and inhibited decay time of sIPSCs. GABA A receptors mediating baseline noise in DGCs were sensitive to allopregnanolone, furosemide, and loreclezole and insensitive to diazepam and zolpidem. These studies demonstrate persistently open GABA A receptors on DGCs with high affinity for GABA, slow desensitization rate, and pharmacological properties similar to those of recombinant receptors containing alpha(4),beta(1), and the delta subunits.

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