4.4 Article

Methanoculleus sediminis sp nov., a methanogen from sediments near a submarine mud volcano

出版社

MICROBIOLOGY SOC
DOI: 10.1099/ijs.0.000233

关键词

-

资金

  1. Ministry of Education, Taiwan, ROC under the ATU
  2. Central Geological Survey, MOEA [99-5226904000-04-03]
  3. Ministry of Science and Technology, Taiwan, ROC [MOST103-3113 M-005-001, MOST104-3113 M-005-001]

向作者/读者索取更多资源

A mesophilic, hydrogenotrophic methanogen, strain S3Fa(T), was isolated from sediments collected by Ocean Researcher I cruise ORI-934 in 2010 near the submarine mud volcano MV4 located at the upper slope of south-west Taiwan. The methanogenic substrates utilized by strain S3Fa(T) were formate and H-2/CO2 but not acetate, secondary alcohols, methylamines, methanol or ethanol. Cells of strain S3Fa(T) were non-motile, irregular cocci, 0.5-1.0 mu m in diameter. The surface-layer protein showed an Mr of 128 000.The optimum growth conditions were 37 degrees C, pH 7.1 and 0.17 M NaCl. The DNA G+C content of the genome of strain S3FaT was 62.3 mol %. Phylogenetic analysis revealed that strain S3Fa(T) was most closely related to Methanoculleus marisnigri JR1(T) (99.3 % 16S rRNA gene sequence similarity). Genome relatedness between strain S3Fa(T) and Methanoculleus marisnigri JR1(T) was computed using both genome-to-genome distance analysis (GGDA) and average nucleotide identity (ANI) with values of 46.3-55.5 % and 93.08 %, respectively. Based on morphological, phenotypic, phylogenetic and genomic relatedness data, it is evident that strain S3Fa(T) represents a novel species of the genus Methanoculleus, for which the name Methanoculleus sediminis sp. nov. is proposed. The type strain is S3Fa(T) (=BCRC AR10044(T)=DSM 29354(T)).

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.4
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据