期刊
PLANT SCIENCE
卷 170, 期 2, 页码 307-313出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.plantsci.2005.08.025
关键词
polyphosphate metabolism; phosphate deficient condition; acidophilic algae; Chlamydomonas; fluorescent microscopy; in vivo P-31 NMR
In order to study the polyphosphate (PolyP) metabolism in the acidophilic alga, the effect of rapid change of external phosphate concentration (so-called Pi stress condition) on the distribution of PolyP in Chlamyclomonas acidophila KT-1 was investigated by fluorescent microscopy, in vivo P-31 NMR spectroscopy, and enzymatic quantification. The PolyP granules inside of C acidophila KT-I cells were observed by microscopy with a fluorescent dye 4',6-diarnidino-2-phenylindole (DAPI). Cells transferred from phosphate (Pi) deficient to Pi rich conditions were contained a large amount of PolyP and an additional new PolyP stain was observed around the cell surface. In comparison to a neutrophilic alga Chlamydomonas reinhardtii C-9, the total amount of PolyP in C. acidophila KT-1 was 2.5 times smaller. The lower content of PolyP in C. acidophila KT-1 was supposed to be attributable to the specific properties of its cell membrane, which limited to incorporate phosphate from the environment. Nevertheless, the trend of degradation and re-synthesis of PolyP in C. acidophila KT-1 was similar to that of C reinhardtii C-9 under Pi stress conditions with absolute PolyP amount being affected by the total phosphate in the cells. (c) 2005 Elsevier Ireland Ltd. All rights reserved.
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