Chryseobacterium soldanellicola sp nov and Chryseobacterium taeanense sp nov, isolated from roots of sand-dune plants

Two Gram-negative, yellow-pigmented bacteria designated PSD1-4(T) and PHA3-4(T), isolated from two sand-dune plant species inhabiting coastal areas in Tae-an, Korea, were subjected to taxonomic investigation. 16S rRNA gene sequence analysis indicated that both isolates should be placed in the genus Chryseobacterium of the family Flavobacteriaceae. The phenotypic properties of the strains were also consistent with their classification into this genus. The levels of 16S rRNA gene sequence similarity between strain PSD1-4(T) and other Chryseobacterium species were 95-2-97-2%; those between PHA3-4(T) and others were 93-7-97-8%. The DNA-DNA relatedness data indicated that strains PSD1-4(T) and PHA3-4(T) were clearly different from the nearest species, Chryseobacterium indoltheticum and Chryseobacterium taichungense. The major fatty acids were 13-methyltetradecanoic acid (iso-C15:0), 3-hydroxy-15-methylhexadecanoic acid (iso-C17:0 3-OH) and omega-g-cis-15-methylhexadecenoic acid (iso-C17:1 omega 9c) for both strains. On the basis of polyphasic taxonomic analysis results, it is evident that each of these strains represents a novel species of Chryseobacterium, for which the names Chryseobacterium soldanellicola sp. nov. (type strain PSD1-4(T) = KCTC 12382(T) = NBRC 100864(T)) and Chryseobacterium taeanense sp. nov. (type strain PHA3-4(T) =KCTC 12381(T) =NBRC 100863(T)) are proposed.