Differential stimulation of muscle protein synthesis in elderly humans following isocaloric ingestion of amino acids or whey protein

To counteract the debilitating progression of sarcopenia, a protein supplement should provide an energetically efficient anabolic stimulus. We quantified net muscle protein synthesis in healthy elderly individuals (65-79 yrs) following ingestion of an isocaloric intact whey protein supplement (WY; n = 8) or an essential amino acid supplement (EAA; n = 7). Femoral arterio-venous blood samples and vastus lateralis muscle biopsy samples were obtained during a primed, constant infusion of L-[ring-H-2(5)]phenylalanine. Net phenylalanine uptake and mixed muscle fractional synthetic rate (FSR) were calculated during the post-absorptive period and for 3.5 h following ingestion of 15 g EAA or 15 g whey. After accounting for the residual increase in the intracellular phenylalanine pool, net post-prandial phenylalanine uptake was 53.4 +/- 9.7 mg phe leg(-1) (EAA) and 21.7 +/- 4.6 mg phe leg(-1) (WY), (P < 0.05). Postabsorptive FSR values were 0.056 +/- 0.004% h(-1) (EAA) and 0.049 +/- 0.006% h(-1) (WY), (P > 0.05). Both supplements stimulated FSR (P < 0.05), but the increase was greatest in the EAA group with values of 0.088 +/- 0.01117% h(-1) (EAA) and 0.066 +/- 0.004% h(-1) (WY), (P < 0.05). While both EAA and WY supplements stimulated muscle protein synthesis, EAAs may provide a more energetically efficient nutritional supplement for elderly individuals. (c) 2005 Elsevier Inc. All rights reserved.