期刊
JOURNAL OF VIROLOGY
卷 80, 期 4, 页码 2013-2018出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/JVI.80.4.2013-2018.2006
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Influenza virus hemagglutinin (HA) is a determinant of virus infectivity. Therefore, it is important to determine whether RA of a new influenza virus, which can potentially cause pandemics, is functional against human cells. The novel imaging technique reported here allows rapid analysis of RA function by visualizing viral fusion inside cells. This imaging was designed to detect fusion changing the spectrum of the fluorescence-labeled virus. Using this imaging, we detected the fusion between a virus and a very small endosome that could not be detected previously, indicating that the imaging allows highly sensitive detection of viral fusion.
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