Lysobacter novalis sp nov., isolated from fallow farmland soil

A novel bacterial strain, designated THG-PC7(T), was isolated from fallow farmland soil in Yongin, South Korea. Cells of strain THG-PC7(T) were Gram-stain-negative, dark yellow, aerobic, rod-shaped and had gliding motility. Strain THG-PC7(T) grew optimally at 25-35 degrees C, at pH 7 and in the absence of NaCl. Comparative 16S rRNA gene sequence analysis identified strain THG-PC7(T) as belonging to the genus Lysobacter, exhibiting highest sequence similarity with Lysobacter ximonensis KCTC 22336(T) (98.7 %) followed by Lysobacter niastensis KACC 11588(T) (95.7 %). In DNA-DNA hybridization tests, DNA relatedness between strain THG-PC7(T) and its closest phylogenetic neighbour L. ximonensis was below 25 %. The DNA G +C content of the novel isolate was determined to be 62.5 mol%. Flexirubin-type pigments were found to be present. The major cellular fatty acids were determined to be iso-C-15: 0, iso-C-16: 0, anteiso-C-15 :0 and iso-C-17 : 1 omega 9c. The major respiratory quinone was identified as ubiquonone-8 (Q8). The predominant polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and an unidentified aminophospolipid. On the basis of results from DNA-DNA hybridization and the polyphasic data, strain THG-PC7(T) represents a novel species of the genus Lysobacter, for which the name Lysobacter novalis sp. nov. is proposed. The type strain is THG-PC7(T)(=KACC 18276(T)=CCTCC AB 2014319(T)).