Isoelectric point determination of proteins and other macromolecules: Oscillating method

A program written in Visual Basic has been developed to calculate the isoelectric point of proteins and other macromolecules bearing acid-basic residues. The pI value can be theoretically calculated with the precision required. The computer automatically supplies a representation of the charge of the protein versus pH values. The corresponding values can also be obtained, on command, in the form of table. (C) 2004 Elsevier Ltd. All rights reserved.