Peripheral inflammation modifies the effect of intrathecal IL-1β on spinal PGE2 production mainly through cyclooxygenase-2 activity.: A spinal microdialysis study in freely moving rats

Acute inflammation induces upregulation of IL-1 beta both at the site of the peripheral inflammation and in the cerebrospinal fluid (CSF). The central increase of IL-1 beta mainly contributes to the development of hypersensitivity. However, the spinal mechanisms for the effects of I in nociceptive transmission are incompletely understood. It is also unknown whether previous sensitization changes IL-1 beta activity, We therefore investigated the dose-effect relationship of intrathecal (i.t.) IL-1 beta oil spinal PGE(2) production in the absence and presence of peripheral formalin inflammation with spinal microdialysis in freely moving rats. The possible involvement of cyclooxygenase (COX) isoforms in the IL-1 beta-mediated spinal PGE(2) production Oil the background of peripheral formalin inflammation was further evaluated with the selective COX-1 and COX-2 inhibitors. We found that the i.t. administration of IL-1 beta, with doses of 1, 2, 8, or 16 ng, increased PGE(2) levels in CSF in a dose-related fashion. This IL-1 beta-evoked PGE(2) release occurred within 30 min after IL-1 beta adiministration, peaked at 30-60 min interval, and returned gradually to the baseline level within 4 h. Peripheral formalin inflammation in the paw induced a more prolonged effect of spinal IL-1 beta with larger PGE(2) releases in the CSF compared with the non-inflammatory state, suggesting that peripheral inflammation enhances central sensitization. The COX-2 inhibitor SC58236 (15mg/kg) reduced the IL-1 beta-mediated PGE(2) increase in CSF by 86% while the COX-1 inhibitor SC58560 (15 mg/kg) had less effect (28%). Our study suggests that mainly the COX-2 enzyme mediates the IL-1 beta-induced increase in spinal PGE(2) in the presence of peripheral formalin inflammation. (c) 2005 International Association for the Study of Pain. Published by Elsevier B.V. All rights reserved.