期刊
JOURNAL OF PROTEOME RESEARCH
卷 5, 期 2, 页码 233-239出版社
AMER CHEMICAL SOC
DOI: 10.1021/pr050268v
关键词
histone; chromatin; post-translational modifications; top down mass spectrometry; electron capture dissociation
资金
- NIGMS NIH HHS [5T32 GM 08276, GM 067193-03] Funding Source: Medline
The basis set of protein forms expressed by human cells from the H2B gene family was determined by Top Down Mass Spectrometry. Using Electron Capture Dissociation for MS/MS of H2B isoforms, direct evidence for the expression of unmodified H2B.Q, H2B.A, H2B.K/T, H2B.J, H2B.E, H2B.B, H2B.F, and monoacetylated H2B.A was obtained from asynchronous HeLa cells. H2B.A was the most abundant form, with the overall expression profile not changing significantly in cells arrested in mitosis by colchicine or during mid-S, mid-G2, G2/M, and mid-G1 phases of the cell cycle. Modest hyperacetylation of H2B family members was observed after sodium butyrate treatment.
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