期刊
TISSUE ENGINEERING
卷 12, 期 2, 页码 337-344出版社
MARY ANN LIEBERT, INC
DOI: 10.1089/ten.2006.12.337
关键词
-
Immunoisolation of pancreatic islets interrupts their vascular connections and results in severe cell hypoxia and dysfunction. This process is believed to be the major obstacle to a successful cure of diabetes by implantation of bioartificial pancreas. Here we describe a new technology for microalga-based, photosynthetic oxygen supply to encapsulated islets, in which a thermophylic strain of the unicellular alga Chlorella was used as a natural photosynthetic oxygen generator. Following determinations of the optimal number of alga cells required for compensation of islet respiration, an appropriate number of islets and algae were co-encapsulated in alginate and perifused with oxygen-free medium at increasing glucose concentrations. No insulin response to glucose was obtained in islets alone, or upon inactivation of photosynthesis by darkness. However, under illumination, photosynthetic-dependent oxygen generation induced higher glucose-stimulated insulin response when compared to normoxic perifusion. Such photosynthetic oxygen generation may have a potential application in development of various bioartificial tissues, in particular the endocrine pancreas.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据