Bradyrhizobium erythrophlei sp nov and Bradyrhizobium ferriligni sp nov., isolated from effective nodules of Erythrophleum fordii

Six slow-growing rhizobial strains isolated from effective nodules of Erythrophleum fordii were classified into the genus Bradyrhizobium based on their 16S rRNA gene sequences. The results of multilocus sequence analysis of recA, glnll and gyrB genes and 16S-23S rRNA intergenic spacer (IGS) sequence phylogeny indicated that the six strains belonged to two novel species, represented by CCBAU 53325(T) and CCBAU 51502(T), which were consistent with the results of DNA DNA hybridization; CCBAU 53325(T) had 17.65-25.59 % relatedness and CCBAU 515021 had 22.69-44.58 % relatedness with five closely related type strains, Bradyrhizobium elkanii USDA 76(T), B. pachyrhizi LMG 24246(T), B. lablabi CCBAU 23086(T), B. jicamae LMG 24556(T) and B. japonicum USDA 6(T). In addition, analysis of phenotypic characteristics and fatty acid profiles also distinguished the test strains from defined species of Bradyrhizobium. Two novel species, Bradyrhizobium erythrophlei sp. nov., represented by the type strain CCBAU 53325(T) (=HAMBI 3614(T)=CGMCC 1.13002(T)=LMG 28425(T)), and Bradyrhizobium ferriligni sp. nov., represented by the type strain CCBAU 51502(T) (=HAMBI 3613(T)=CGMCC 1.13001(T)), are proposed to accommodate the strains.