期刊
BLOOD
卷 107, 期 3, 页码 924-930出版社
AMER SOC HEMATOLOGY
DOI: 10.1182/blood-2005-05-2140
关键词
-
类别
资金
- NCI NIH HHS [CA 46592] Funding Source: Medline
- NIAMS NIH HHS [1 P30 AR48310, AR20557] Funding Source: Medline
Recent advances have increased the purity of hematopoietic stem cells (HSCs) isolated from young mouse bone marrow. However, little attention has been paid to the purity of HSCs from other contexts. Although Thy-1(low)Sca-1(+)Lineage(-)c-kit(+) cells from young bone marrow are highly enriched for HSCs (1 in 5 cells gives long-term multilineage reconstitution after transplantation into irradiated mice), the same population from old, reconstituted, or cytokine-mobilized mice engrafts much less efficiently (11 in 78 to I in 185 cells gives long-term multilineage reconstitution). To test whether we could increase the purity of HSCs isolated from these contexts, we examined the SLAM family markers CD150 and CD48. All detectable HSCs from old, reconstituted, and cyclophosphamide/G-CSF-mobilized mice were CD150(+)CD48(-), just as in normal young bone marrow. Thy-1(low)Sca1(+)Lineage(-)c-kit(+) cells from old, reconstituted, or mobilized mice included mainly CD48(+) and/or CD150(-) cells that lacked reconstituting ability. CD150(+)CD48(-)Sca-1(+)Lineage(-)c-kit(+) cells from old, reconstituted, or mobilized mice were much more highly enriched for HSCs, with 1 in 3 to 1 in 7 cells giving long-term multilineage reconstitution. SLAM family receptor expression is conserved among HSCs from diverse contexts, and HSCs from old, reconstituted, and mobilized mice engraft relatively efficiently after transplantation when contaminating cells are eliminated.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据