期刊
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
卷 384, 期 4, 页码 939-945出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-005-0238-8
关键词
enantiomer separation; polysaccharide carbamate phase; specific rotation; circular dichroism; tetrahydropalmatine; tetrahydroberberine
HPLC methods have been developed for chiral resolution of the enantiomers of dl-tetrahydropalmatine (THP) and dl-tetrahydroberberine (THB), two active constituents of Corydalis yanhusuo W.T. Wang. On the analytical scale, good baseline separation of the enantiomers was achieved using cellulose tris(3,5-dimethylphenylcarbamate) chiral stationary phases in both normal-phase and polar organic modes. Validation of the analytical methods, including linearity, limits of detection, recovery, and precision, and semipreparative resolution of dl-THP and dl-THB, were achieved with methanol as mobile phase, without any basic additives, in polar organic mode using cellulose tris(3,5-dimethylphenylcarbamate) chiral stationary phases. On the semipreparative scale, small quantities of the individual enantiomers of THP and THB were isolated for study of the chiroptical properties of the individual enantiomers.
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